Comparison of two- and three-dimensional sonography for the prediction of the extrathyroidal extension of papillary thyroid carcinomas

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Abstract

INTRODUCTION

Recently, there has been a marked increase in the incidence of small-sized thyroid cancer [1-3], and an accurate determination of the necessary extent of surgery has become a matter of concern. The presence of extrathyroidal extension changes the scope of surgery from thyroid lobectomy to a total or near-total thyroidectomy in the case of a unifocal papillary thyroid carcinoma that is 1 cm or smaller in size and clinically node-negative in a low-risk patient [4,5]. Additionally, papillary thyroid carcinomas, and most follicular cancers sized 4 cm or less that do not have evidence of cervical nodal metastases, may not require prophylactic central neck dissection in the absence of extrathyroidal extension [4,5].

Neck ultrasonography (US) has emerged as an essential preoperative staging modality for patients with a malignant thyroid nodule [4]. Several previous studies have evaluated the preoperative characteristics of US that may be predictive of the extrathyroidal extension of papillary thyroid cancer. These studies suggested the use of different criteria including the presence of contact itself [6] or various degrees of contact between the papillary thyroid cancer and the adjacent capsule [7,8]. A previous study conducted by our group revealed that contact with the capsule is predictive of extrathyroidal extension of papillary thyroid cancer and that contact in combination with protrusion into adjacent tissues is a stronger independent predictive factor [9].

Because conventional two-dimensional (2D) US has limitations regarding assessment in the coronal plane, some authors have proposed that evaluation with three-dimensional (3D) US is necessary [8,9]. During preoperative staging of endometrial cancer, cervical cancer, prostate cancer, and bladder cancer, 3D US has been shown to be a useful tool for the detection of the invasion or extension of cancers into adjacent tissues [10-14]. However, to the best of our knowledge, only one recent study has evaluated the diagnostic performance of 3D US in the assessment of extrathyroidal extension of thyroid cancer [15].

This study aimed to compare the diagnostic accuracy of real-time 2D US data with that of static 3D US data for the prediction of the extrathyroidal extension of papillary thyroid cancer into the adjacent capsule according to presence of contact and protrusion in an attempt to determine the usefulness of 3D US data rendered using tomographic ultrasound imaging (TUI).

METHODS

Preoperative ultrasound examinations and image assessment

Both 2D and 3D images were obtained using one scanner (LOGIQ E9, GE Healthcare, Milwaukee, WI, USA) operated by three endocrinologists (authors JHK, BGC, and SSK) according to their duty schedule. The preoperative 2D US examination was performed using 3 to 11 MHz and 4 to 15 MHz linear transducers, and the 3D US volume data was acquired using a 6 to 8 MHz real-time four-dimensional linear transducer. The 3D volumes were scanned using automatic capture for the fine needle aspiration-proven thyroid papillary cancers and any additional suspicious malignant nodules.

The 2D US images were interpreted immediately following examination by the US operator for presence of contact with the thyroid capsule and presence of protrusion into adjacent structures. The 3D US data were stored and later rendered using the TUI method with a slice interval of 0.5 to 0.8 mm depending on the nodule size (Fig. 1). Two additional endocrinologists who had experience in thyroid US (authors YSY and SSK) and who were blind to the 2D US results reviewed the TUI-rendered 3D US images to determine the contact and protrusion of the nodules. Both the 2D and 3D data reviewers were blind to the pathological results.

Figure 1.

Three-dimensional ultrasonograms of a representative presence of both contact and protrusion (C1P1) papillary thyroid carcinoma rendered using tomographic ultrasound imaging. A series of slices (0.5 mm apart) in the transverse plane (A), the longitudinal plane (B), and the coronal plane (C). The lesion shows contact and protrusion in the transverse (white arrows) and the longitudinal plane (arrowhead). After surgery, pathologic finding was negative for extrathyroidal extension.

Presence of contact was defined as the absence of grossly visible intervening normal thyroid tissue between the nodule and the adjacent thyroid capsule [8,9]. If a nodule exhibited contact, it was further assessed to determine whether it protruded into adjacent structures. The nodules were first categorized according to the presence of contact: absence of contact (C0) (Fig. 2) and presence of contact (C1). And then C1 nodules were further categorized with regard to the presence of protrusion: presence of contact without protrusion (C1P0) (Fig. 3) and presence of protrusion in addition to contact (C1P1) (Fig. 4).

Figure 2.

A representative absence of contact (C0) papillary thyroid carcinoma. Traverse (A) and longitudinal (B) sonograms from two-dimensional ultrasonography demonstrate the absence of contact by the papillary thyroid carcinoma. Tomographic ultrasound imaging rendered images of three-dimensional ultrasonography in the transverse (C), longitudinal (D), and coronal (E) planes are corresponding with the two-dimensional ultrasonographic findings. After surgery, pathologic report was also negative for extrathyroidal extension.

Figure 3.

A representative presence of contact without protrusion (C1P0) papillary thyroid carcinoma. Transverse (A) and longitudinal (B) sonograms from two-dimensional ultrasonography demonstrating that the carcinoma is in contact with the thyroid capsule but does not protrude into adjacent structures (white arrowhead). Tomographic ultrasound imaging rendered images of three-dimensional ultrasonography in the transverse (C), longitudinal (D), and coronal (E) planes show that the papillary carcinoma is in contact with the capsule in the transverse and longitudinal planes (black arrowheads) without definite protrusion. In the coronal plane, the lesion is also considered in contact (black arrow) with the adjacent capsule (white arrows). Pathologic extrathyroidal extension was confirmed.

Figure 4.

A representative presence of both contact and protrusion (C1P1) papillary thyroid carcinoma. The carcinoma is in contact with the thyroid capsule and protrudes into adjacent structures in the transverse (A) and longitudinal (B) planes on sonograms from two-dimensional ultrasonography (white arrowheads). Tomographic ultrasound imaging rendered images from three-dimensional ultrasonography also demonstrate that the carcinoma is in contact with and protrudes in the transverse (C) and longitudinal (D) planes (black arrowheads). In the coronal (E) plane, the lesion is in contact with the capsule (white arrows), but protrusion is not definite (black arrow). Final pathologic finding was negative for extrathyroidal extension.

RESULTS

The demographic and clinicopathological characteristics of the 61 patients included in this study are presented (Table 1). The mean age was 48.7 ± 13.1 years (range, 17 to 75). Of these, 19 patients (31.1%) were younger than 45 years of age, 20 (32.8%) had two or more papillary thyroid carcinomas, and 25 (41%) had lymph node metastasis; none of the patients had distant metastasis at the time of diagnosis of thyroid cancer. The mean interval between preoperative 2D and 3D US was 6.7 days (range, 0 to 83). The preoperative 2D US characteristics of the papillary thyroid cancers are presented in Table 2. The most commonly observed preoperative characteristics of the cancers on 2D US were solid (81.4%), markedly hypoechoic (45.7%), hypoechoic (42.9%), irregular shape (60.0%), ill-defined margin (87.1%), and microcalcification with or without macrocalcification (55.7%). The most common location was the middle third (48.6%) of the thyroid, and, as measured by 2D US, the mean size for all cancers regardless of extrathyroidal extension was 0.90 ± 0.36 cm. The mean size of cancers with extrathyroidal extension was 0.94 ± 0.20 cm, which was not significantly different from the size of cancers without extrathyroidal extension (0.89 ± 0.40 cm), based on the t test (p = 0.483).

Table 1.

Patient demographic and clinicopathological characteristics

Table 2.

Two-dimensional ultrasonographic characteristics of papillary thyroid carcinomas

According to the histopathological reports, 55 cancers (78.6%) were micropapillary thyroid carcinoma, and extrathyroidal extension was present in 17 (24.3%) of the 70 carcinomas. Of these 17 cases, 15 (88.2%) exhibited minimal extrathyroidal extension, and the other two had invaded the recurrent laryngeal nerve; 12 of the 17 cases (70.6%) were microcarcinomas.

In terms of contact, 3D and 2D C1 cases were significantly associated with extrathyroidal extension (p = 0.003 and p = 0.007, respectively) (Table 3). However, in the further analysis with regard to protrusion, 2D C1P1 cases in the 2D C1 subgroup as well as 3D C1P1 cases in the 3D C1 subgroup showed no significant association (p = 0.313 and p = 0.795, respectively) (Table 4). Evaluation of protrusion using 3D US in the 2D C1 subgroup also did not show significant difference in the prediction of extrathyroidal extension (p = 0.772). For 3D C1 cases, the sensitivity and specificity for predicting extrathyroidal extension were 94.1% and 45.3%, respectively, and for 2D C1 cases, the sensitivity and specificity were 94.1% and 41.5%, respectively (Table 5). There were no significant differences in sensitivity and specificity between the 2D C1 and 3D C1 cases (p = 1.000 and p = 0.754, respectively).

Table 3.

Associations between ultrasonographic presence of contact and extrathyroidal extension of papillary thyroid carcinomas

Table 4.

Associations between coexistence of protrusion with contact and extrathyroidal extension of papillary thyroid carcinomas

Table 5.

Diagnostic performance of ultrasonographic presence of contact for predicting extrathyroidal extension of papillary thyroid carcinomas

In terms of contact, there was a discrepancy between the 3D and 2D US findings for 10 of the 70 papillary thyroid carcinomas: four cancers showed presence of contact on 3D US (3D C1) but not on 2D US (2D C0), and six cancers showed presence of contact on 2D US (2D C1) but not on 3D US (3D C0) (Table 6). The additional 3D US assessments employed in conjunction with the standard 2D US examination for presence of contact did not significantly differ for the prediction of extrathyroidal extension in 2D C0 and 2D C1 cases (p = 1.000 and p = 0.060, respectively).

Table 6.

Diagnostic value of additional 3D ultrasonography following routine 2D ultrasonography using contact to predict the extrathyroidal extension of papillary thyroid carcinomas

DISCUSSION

The preoperative staging of thyroid cancer is important in order in determining the extent of surgery required [4,5], especially in the case of small-sized thyroid cancer. US is an essential tool during the preoperative staging of thyroid cancer to assess size, multifocality, and cervical lymph node metastasis [4,5] as well as to predict extrathyroidal extension [6-9,17,18].

A number of previous studies have investigated preoperative 2D US characteristics that may be predictive of extrathyroidal extension of papillary thyroid cancer, and various sonographic criteria have been suggested. Kwak et al. [8] proposed contact with the capsule by > 25% of the nodule perimeter could be a useful criteria for papillary microcarcinoma with sensitivity of 65.2%, specificity of 81.8%, and the accuracy (Az) of 0.735. Park et al. [7] used a criteria of > 50% of the capsular abutting diameter relative to the whole tumor diameter, and reported sensitivity of 85.3%, specificity of 70.0%, and accuracy of 74.5%. A previous study by our group evaluated 354 patients with papillary thyroid carcinomas 2 cm or smaller in size according to the presence of contact and the presence of protrusion into adjacent structures [9]. A multivariate analysis revealed that the presence of contact is predictive of extrathyroidal extension and that its coexistence with protrusion has an even greater predictive value. A recent study applied a more extensive criteria including either the presence of contact or the presence of protrusion, and the sensitivity, specificity, and diagnostic accuracy were 83.3%, 68.9%, and 75.9%, respectively [18].

However, those studies employed conventional 2D US, which has a reported false-negative rate of 14.7% to 52.0% [7-9,18]. Some researchers have suggested that 3D US may provide more information regarding the relationship between thyroid cancers and the adjacent capsule in the coronal plane, and it may therefore have greater predictive value regarding extrathyroidal extension than 2D US can provide. In fact, 3D US assessment has been reported to be useful for predicting the invasion or extension of endometrial cancer [10], cervical cancer [11], prostate cancer [12], and bladder cancer [13,14] during the preoperative staging.

In assessments using 3D US, the volume data are scanned, stored, and later processed according to an imaging-rendering algorithm such as the multiplanar reformation mode which renders the initial 2D images in the longitudinal, transverse, and coronal planes simultaneously [19]. Various other imaging-rendering algorithms can be combined to acquire an optimized image [20]. TUI is a novel 3D post-processing technique that presents the volume data as a set of contiguous sequential slices in the transverse, longitudinal, and coronal planes in a manner similar to that of magnetic resonance or computed tomography images [20]. In a study of deep endometriosis, the TUI rendering technique was considered useful for determining the extension and depth of infiltration in the coronal plane as well as evaluating the relationship of the tumor with adjacent structures [21].

To our knowledge, only one recent study reported the diagnostic performance of 3D US in the preoperative prediction of extrathyroidal extension of papillary thyroid cancer [15]. The sonographic criteria of extrathyroidal extension was either > 25% perimeter of the tumor contact with the capsule or the presence of protrusion. In the comparison with diagnostic accuracy of 2D US, that of 3D US alone was not significantly different whereas that of the combined set of 2D and 3D images was significantly higher. Sensitivity, specificity, and accuracy of combined set were 87.8%, 29.6%, and 67.9%, respectively, and those of 2D US were 78.2%, 27.2%, and 60.8%, respectively. Since the study included papillary thyroid carcinomas that were suspected to be in contact with the capsule on 2D US, the prevalence of pathologic extrathyroidal extension was as high as 65.8%, which might have affected diagnostic performance. Furthermore, retrospective review of static 2D images could have limitation in determining the degree of contact and might partly disagree with the dynamic examination.

The present study compared the diagnostic accuracy of preoperative 2D and 3D US assessments of papillary thyroid cancers 2 cm or smaller in size for the prediction of extrathyroidal extension. The TUI rendering method was applied to the 3D volume data, and the sonographic criteria for extrathyroidal extension included the presence of contact either with or without protrusion. The 3D US assessment did not significantly differ from the 2D US assessment in terms of predicting extrathyroidal extension; the presence of contact with the thyroid capsule was significantly associated with extrathyroidal extension on both the 2D and 3D findings. Compared with previous studies using 2D US, the present study had a lower false-negative rate but a higher false-positive rate for 2D C1 and 3D C1 cases. Discrepancies between 2D and 3D US assessments concerning the interpretation of contact were found for 10 of 70 papillary thyroid carcinomas, but the additional information provided by the 3D US data did not lead to a significant difference. Although there was no statistical significance, none of the 3D C0 cases in the 2D C1 subgroup had extrathyroidal extension so that additional 3D US examination could have the possibility to be helpful to exclude extrathyroidal extension.

Even though we expected that 3D US assessment would help to acquire more sensitive results compared with 2D US examination by providing additional information in the coronal plane, 3D C1 showed a tendency to be more specific rather than sensitive in predicting extrathyroidal extension compared with 2D C1. One possible reason is that images in the coronal plane of 3D US have relatively poor resolution, and are not as familiar to the examiner as with 2D images. The coronal plane of 3D US is a virtual reconstruction completed by the computer. Consequently, images in the coronal plane have relatively poor resolution, which sometimes obscure and complicate evaluation in the coronal plane [19,22]. Another possible reason is that the 2D US data were assessed in real time and interpreted immediately, whereas the 3D data were stored before being rendered and then retrospectively assessed. This difference in the timing of assessment reflects real conditions in the clinical field. Compared with retrospective analysis of stored 2D data, real time assessment could be more variable according to the observers and cannot be re-evaluated afterwards. On the other hand, US examiners can make decision on the basis of dynamic assessment of spatial relationship so that real time interpretation could be more preferred especially when the degree of contact should be determined as in some cases of the previous studies using 2D US.

Unlike our previous study, the concomitant presence of protrusion in addition to contact was not significantly associated with extrathyroidal extension in either the 2D or the 3D US data. Further analysis of the presence of protrusion on 3D US in the 2D C1 subgroup did not show significant association as well. Much lower prevalence of extrathyroidal extension and different examiners from the previous study might be the cause of dissimilarity.

The present study has several limitations. First, the prevalence of extrathyroidal extension was relatively low considering that the rate of extrathyroidal extension observed in previous studies was 36.2% to 55.1% [6-9,17,18]. Although the present study included papillary thyroid carcinomas larger than 1 cm, extrathyroidal extension was reported in only 17 of 70 papillary thyroid carcinomas (24.3%), and just two of these grossly invaded the recurrent laryngeal nerve. This may reflect a recent trend toward the increased detection of early-stage thyroid cancer. Second, our study subjects had higher proportion of female. The male to female ratio in the incidence of thyroid cancer has been reported about 1:3.5 to 1:5 [3,23,24]. Men are probably related to more advanced disease at presentation: larger primary tumor size, higher rates of extrathyroidal extension, regional lymph node metastasis, and distant metastasis in all age groups [24]. In our study, only five male patients with six thyroid carcinomas were included and the male to female ratio was as small as 1:11.2. None of these male patients had extrathyroidal extension, which might be a contributing factor to a lower prevalence of extrathyroidal extension in our study group. Third, contact was categorized in terms of presence or absence. In a previous study using conventional 2D US, the criteria for contact alone had higher sensitivity but lower specificity and diagnostic accuracy for predicting extrathyroidal extension compared with criteria that required more than 25% contact [8]. Categorization based on the degree of contact should be further evaluated in the future study using 3D US. Fourth, the present study used only the TUI method to analyze the relationship between the thyroid cancer and the adjacent capsule. For better visualization of the images in the coronal plane, the concomitant use of other post-processing algorithms may be needed. A thin-slice smooth-surface rendering algorithm was found to provide a more detailed visualization of thyroid nodules such that twice the number of thyroid nodules in the coronal plane could be analyzed using this technique compared with the multiplanar reformation mode [20,22]. The volume contrast imaging method also has been reported to enhance the tissue demarcation of endometrial cancer [10] and could be a useful method for the assessment of thyroid cancer as well [20]. Fifth, we did not evaluate the interobserver or intraobserver variability in interpreting the presence of contact and protrusion on 2D and 3D US. To our knowledge, two studies have evaluated interobserver agreement of US for the assessment of extrathyroidal extension of thyroid cancer, and fair to moderate agreement with 2D US [25,15] and moderate agreement with 3D US [15] were reported. Relatively inferior interobserver agreement may be partly because the criteria for assessing extrathyroidal extension has not been clearly established in comparison with other criteria such as that for defining metastatic lymph node [25]. In addition, since our study assessed with 2D US in real time manner whereas with 3D US in retrospective review, interpretations could have been even more variable. Sixth, we included papillary thyroid carcinomas 2 cm or smaller size so that the diagnostic value of 3D US in comparison with 2D US for larger size cancers could not be determined in the present study.

In conclusion, 3D US data rendered with the TUI algorithm alone and then analyzed based on the presence or absence of contact do not seem to have remarkable advantage over evaluation with real-time 2D US for the prediction of the extrathyroidal extension of papillary thyroid carcinoma. However, in the cases with presence of contact on 2D US, further assessment with 3D US could be marginally helpful to exclude extrathyroidal extension. A combination of other reconstruction algorithms of 3D volume data and categorization based on the degree of contact should be further evaluated in the future.

KEY MESSAGE

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