INTRODUCTION
Evaluation of not only anatomic obstructive lesions of the coronary artery but also the functional significance of the lesions is important when determining the treatment strategy for ischemic heart disease [
1,
2]. Revascularization of reversible myocardial ischemia based on thallium single-photon emission computed tomography (SPECT) has been used to improve the clinical outcomes of patients [
1], although medical therapy has shown a treatment efficacy comparable to that of revascularization in small myocardial ischemia lesions involving less than 10% of the left ventricular (LV) myocardium [
3]. Therefore, the use of functional imaging to assess myocardial ischemia is an emerging topic. Stress myocardial computed tomography perfusion (CTP) is one of the diagnostic tools used to detect myocardial ischemia, and is attracting attention because of its accuracy, convenience, and non-invasiveness [
4].
Myocardial contrast enhancement can be evaluated by visual and quantitative analysis. Although visual analysis is the principal form of analysis used, an objective evaluation of CTP can be conducted by quantitative assessment of the ischemic burden of the myocardium [
5]. Multiplanar reconstructions and minimum intensity projections of the LV myocardium have been used to compare myocardial perfusion defects with the level of perfusion of the normal myocardium. The transmural perfusion ratio (TPR) is derived from a comparison of the endomyocardial attenuation to that of the subepimyocardium [
6]. The TPR shows good correlation with SPECT and invasive coronary angiography [
7]. Both visual and quantitative analyses are based on an assessment of the attenuation of the contrast-enhanced LV myocardium; however, these methods can be disturbed by certain variables, including anatomical attenuation, gender-specific differences, different computed tomography (CT) scanner-derived factors, and CT artifacts [
8-
11]. One study has shown that the LV perfusion in the rest phase measured in asymptomatic patients was significantly higher in women than in men [
10]. In 64-detector row CT, the normal myocardial perfusion in the LV myocardial septum showed higher attenuation than in the lateral and inferior walls [
9]. Thus, in the present study, we aimed to identify the different attenuations of the LV myocardium according to demographic differences, and the myocardial segment, using a second-generation dual-source CT scanner in patients with normal or minimal coronary artery disease on computed tomography angiography (CTA).
METHODS
Patients
From a multicenter static CTP registry of dual-source CT, which is a prospective registry of all patients who underwent CTP for the evaluation of chest pain, we retrospectively recruited 113 patients with normal CTP on visual assessment and minimal (< 20% diameter stenosis) or no coronary artery disease on CTA.
CT imaging
Stress-rest myocardial CTP protocols were performed using a second-generation dual-source CT scanner (Definition Flash, Siemens, Forchheim, Germany). Adenosine (Denosine, BCWP, Seoul, Korea) infusion (140 mg/kg/min for 5 minutes) was performed with retrospective electrocardiography-gated acquisition (
Fig. 1). A bolus of iodinated contrast material (60 to 70 mL, iomeprol; Iomeron 400, Bracco, Milan, Italy) was injected at a rate of 4.0 mL/sec. Then, 40 mL of a saline chaser was given. Ten minutes after the infusion of adenosine was discontinued, isosorbide dinitrate (Isoket Spray, UCB Pharma, Monheim, Germany) was sprayed into the oral cavity of the patient. Rest myocardial CTP was performed using the same protocol as that used for stress myocardial CTP. To reduce the radiation dose, the dose-pulsing window was reduced to 60% to 75% if the heart rate decreased to less than 70 beats/min after the adenosine infusion was stopped. The tube current-time product was modified in accordance with the body size of the patient. A smooth reconstruction kernel (B10f) was applied for perfusion imaging in both stress and rest CT data.
Quantitative analysis of CTP
LV myocardial attenuation was measured and analyzed using customized software (A-view Cardiac, Asan Medical Center, Seoul, Korea). The mid-diastolic phase of the CT data was used for the analysis. After semiautomatic segmentation of the LV myocardium, the mean attenuations of the 16 myocardial segments were measured at both stress (Density
stress) and rest (Density
rest) phases (
Fig. 2). TPR was calculated for each segment as the ratio of the subendocardial attenuation density to the subepicardial attenuation on short-axis images of the LV myocardium [
5]. Myocardial perfusion reserve index (MPRI) was calculated as the percentage difference in attenuation between stress and rest myocardial CTP images in each segment, as follows: (Density
stress − Density
rest) / Density
rest × 100%.
Statistical analysis
Continuous variables are presented as the mean ± standard deviation. Using Student t test, the myocardial attenuations in all segments were compared according to the anterior, septal, inferior, and lateral; apical, mid, and basal; and left anterior descending artery, right coronary artery, and left circumflex artery territories. Pearson correlation was used to identify any clinical factors related to the differences in myocardial attenuation. To evaluate gender differences in myocardial attenuation, Student t test was used. Univariate and multivariable regression analyses were used to evaluate the relationship between the myocardial attenuation and clinical variables such as age, body mass index (BMI), and Framingham heart score (FHS). A p value less than 0.05 was regarded as statistically significant. All statistical analyses were performed using commercially available software SPSS version 22.0 (IBM Co., Armonk, NY, USA).
DISCUSSION
The findings of the present study indicate significant differences in the quantitative parameters of stress myocardial CTP in healthy subjects without coronary artery disease, according to geometric and demographic variables. The lateral wall of the LV myocardium showed a significantly higher attenuation and the basal level of the LV myocardium showed a lower attenuation than that shown by the other levels. The TPR was also higher in the lateral wall than in the other walls and was significantly lower at the basal level. However, there were no significant differences in the MPRI of the myocardial walls. In terms of gender-specific differences, myocardial attenuation was significantly higher in women than in men. By multivariable analysis, age, BMI, and FHS were significantly associated with myocardial attenuation. By considering the normal variabilities in the quantitative parameters of the CTP derived from the influence of these geographic and demographic parameters, confusion between true ischemia and normal variation can be prevented, and the diagnostic performance of CTP can be improved.
The results of our present study are in accordance with those of previous studies that reported variabilities in myocardial attenuation in CTP according to geometric and sex parameters [
9,
10,
12]. However, the reasons for these variabilities are unclear. One possible explanation is that the BMI of women is lower than that of men. BMI is independently related to myocardial attenuation. Considering the differences in body size and blood volume between genders, a lower BMI and lower blood volume may cause faster circulation in the myocardium, resulting in high myocardial attenuation. We can presume that the reason for the differences in the myocardial perfusion in CTP between the sexes in our current analysis also explains the differences in the pathophysiology and clinical presentation of ischemic heart disease between the sexes [
13]. Similarly, estrogen affects the vascular tone in women [
14]. In a previous report that adjusted for patient size and body surface area, no difference was observed in myocardial enhancement in CTP according to gender [
9]. Decreased contrast e nhancement with an increase in BMI is a well-known phenomenon associated with obesity [
15]. Thus, adjustment of the amount of contrast medium might be required for consistent enhancement of the myocardium, with consideration of the age, sex, and BMI of the patient. Regarding FHS, which showed a negative relationship with myocardial attenuation by multivariable analysis, we postulate that the presence of microvascular lesion that cannot be detected by CTA would affect the myocardial perfusion.
In a previous study, Crossett et al. [
12] have reported that the lateral wall showed significantly lower attenuation in the normal LV myocardium in CTP. However, they measured LV attenuation by using a rectangular region of interest and did not follow the American Heart Association 17-segment model. Moreover, they did not measure the attenuation differences between the subendocardial, mid-myocardial, and subepicardial layers. As we included the entire LV myocardium and used dedicated software, the differences in our results from those of the previous study might be expected.
The present study had several limitations. First, we did not correlate the CTP results with those of other functional imaging modalities such as SPECT or stress echocardiography. Therefore, the perfusion pattern in individuals with a normal coronary artery may not be applicable to other perfusion imaging results. Second, our results might not be applicable to those of other CT scanners with low temporal resolution or other CTP protocols such as the rest-first protocol. However, our findings of normal differences in the geometric and demographic variables will help in the interpretation of CTP results. Third, we did not consider whether β-blocker use would affect coronary artery blood flow and, thus, myocardial perfusion. Fourth, our study is a retrospective analysis, which may have resulted in a selection bias. Older individuals can be expected to have a greater degree of diastolic dysfunction, which could result in microvascular dysfunction and less hyperemia on adenosine, manifesting as a lower myocardial attenuation on CTP.
Despite these limitations, we included a large study population and evaluated the variability of myocardial attenuation on CTP. Moreover, via a comparison of stress and rest myocardial attenuation, TPR and MPRI values in the normal CTP were thoroughly evaluated. Notably, the TPR also showed geometric and demographic variability. However, the MPRI did not show any significant variability. As the MPRI has been shown to be useful in other modalities such as cardiac magnetic resonance [
16], further studies of its usefulness in CTP would be worthwhile. If the MPRI is shown to be effective in the evaluation of myocardial perfusion, its features could conceivably be used in the assessment of CTP without the need to consider geographic and demographic factors.
In conclusion, the lateral wall and basal level of the LV myocardium shows significantly different quantitative parameters from those of the other regions. Myocardial attenuation can vary with the gender, age, BMI, or FHS of the patient. This information may be useful for the assessment of myocardial perfusion defects in CTP.
KEY MESSAGE
1. Stress myocardial computed tomography perfusion (CTP) in patients without coronary artery disease shows higher quantitative parameter values (myocardial attenuation, transmural perfusion ratio [TPR]) in the lateral wall of the left ventricular (LV) myocardium and lower quantitative parameter values (myocardial attenuation, TPR, myocardial perfusion reserve index) at the basal level of the LV myocardium.
2. Stress myocardial CTP in patients without coronary artery disease shows higher attenuation of the LV myocardium in women than in men.
3. There is a significant correlation between the attenuation of the LV myocardium and certain clinical variables, namely, age, body mass index, and Framingham cardiovascular risk score.
4. Consideration of normal variabilities in the quantitative parameters of stress myocardial CTP caused by these geographic and demographical parameters will be useful in the assessment of CTP.